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• The Photoreduction of Nicotinamide-Adenine Dinucleotide by C...

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• Noninvasive Auto-Photoreduction Used as a Tool for Studying ...

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Abstract

The published reports of flash-induced absorbance changes in the 680–690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whether the fluorescence yield changes, which accompany the photoreduction of C-550, might influence the measurements of chlorophyll bleaching is examined. The responses attributed to P-680 and their relationship to C-550 indicate that, if the absorbance measurements are valid, P-680 probably functions as the primary electron donor to PSII rather than as a photochemical sensitizer of the primary redox reaction.