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• Two-Photon Absorption of Bacteriorhodopsin: Formation of a R...

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• Femtosecond Spectroscopic Observations of Initial Intermedia...

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Abstract

A “complete” and quantitative kinetic model for the states and transitions of the barnacle visual pigment in situ has been constructed from intracellular recordings of the early receptor potential responses to long light pulses. The model involves two stable and four thermolabile states and 10 photochemical, thermal, and metabolic transitions among them. The existence of each state and transition is demonstrated by qualitative examination of the response resulting from a carefully chosen experimental paradigm (combination of intensity, duration, and wavelength of adaptation and stimulation). Quantitative examination of the same responses determines all of the model transition rates, but only puts constraints on the state dipole moments. The latter are determined, and the former refined, by quantitative comparison of the predictions of the complete model with the responses to a set of paradigms chosen to involve as many states and transitions as possible. The fact that good fits can be obtained to these responses without further modification of the model supports its completeness.