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Abstract | Full Text | PDF (609 kb) - …more
Copyright © 1975 The Biophysical Society. All rights reserved.
Biophysical Journal, Volume 15, Issue 8, 753-763, 1 August 1975
doi:10.1016/S0006-3495(75)85852-8
Research Article
Iontophoresis of cyclic AMP
M.H. Cohen, D.J. Drage and A. Robertson
Abstract
The design, calibration, and operation of a source of controlled amounts of cyclic AMP (c-AMP) are described. Typically, 1.5 s pulses containing 10(10)-10(-12) molecules of c-AMP can be delivered to a region about 10 mum in diameter on an agar plate. The resulting concentration profiles are given as functions of distance and time. The diffusion coefficient of c-AMP in agar was measured to be 0.97 times 10(-5) cm2-s-1 at 21 degrees C.
