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Copyright © 1975 The Biophysical Society. All rights reserved.
Biophysical Journal, Volume 15, Issue 8, 785-794, 1 August 1975

doi:10.1016/S0006-3495(75)85855-3

Research Article


Number fluctuation spectroscopy of motile microorganisms

D.W. Schaefer and B.J. Berne


Abstract

A random-walk model of motility is used to predict the dynamics of fluctuations in the number of particles in a small observation volume. The results show that number fluctuations provide a measure of the mean swimming speed as well as the persistence length. Experimental light-scattering results are presented for three strains of Escherichia coli whose motion appears random-walk in nature. For the strain with th elongest persistence length, excellent agreement is found that theoretical predictions. For the more erratic strains, however, the shape of the measured scattered light intensity correlation functions indicates the presence of a contribution due to orientational fluctuations.