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Biophys. J. BioFAST: First Published June 20, 2008. doi:10.1529/biophysj.108.132019
© 2008 by the Biophysical Society.

A more recent version of this article appeared on September 15, 2008.
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CELL BIOPHYSICS

Dielectric Cytometry with Three-Dimensional Cellular Modeling

Yoichi Katsumoto 1*, Yoshihito Hayashi 2, Ikuya Oshige 2, Shinji Omori 2, Noriyuki Kishii 2, Akio Yasuda 2 and Koji Asami 3

1 SONY Corporation
2 Sony Corporation
3 Kyoto University

* To whom correspondence should be addressed. E-mail: youichi.katsumoto{at}jp.sony.com.

Submitted on February 22, 2008
Revised on March 31, 2008
Accepted on 29 May 2008


  Abstract
We have developed an efficient method to determine the electric parameters (the specific membrane capacitance Cm and the cytoplasm conductivity {kappa}i) of cells from their dielectric dispersion. Firstly, a limited number of dispersion curves are numerically calculated for a three-dimensional cell model by changing Cm and {kappa}i, and their amplitudes {Delta}{epsilon} and relaxation times {tau} are determined by assuming a Cole-Cole function. Secondly, regression formulas are obtained from the values of {Delta}{epsilon} and {tau} and then used for the determination of Cm and {kappa}i from the experimental {Delta}{epsilon} and {tau}. This method was applied to the dielectric dispersion measured for rabbit erythrocytes (discocytes and echinocytes) and human erythrocytes (normocytes) and provided reasonable Cm and {kappa}i of the erythrocytes and excellent agreement between the theoretical and experimental dispersion curves.

Key Words: Cytoplasm conductivity, Dielectric relaxation, Finite difference method, Interfacial polarization, Membrane capacitance, Red Blood Cell






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Copyright © 2008 by the Biophysical Society.