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Dielectric Cytometry with Three-Dimensional Cellular Modeling

Yoichi Katsumoto ^*, Yoshihito Hayashi ^*, Ikuya Oshige ^*, Shinji Omori ^*, Noriyuki Kishii ^*, Akio Yasuda ^* and Koji Asami 

^* Life Science Laboratory, Materials Laboratories, Sony Corporation, Sony Bioinformatics Center, Tokyo Medical and Dental University, Tokyo, Japan; and Laboratory of Molecular Aggregation Analysis, Division of Multidisciplinary Chemistry, Institute for Chemical Research, Kyoto University, Kyoto, Japan

Correspondence: Address reprint requests to Yoichi Katsumoto, Tel.: 81-3-5803-4791; Fax: 81-3-5803 4790; E-mail: Youichi.Katsumoto{at}jp.sony.com.

We have developed what we believe is an efficient method to determine the electric parameters (the specific membrane capacitance C_m and the cytoplasm conductivity _i) of cells from their dielectric dispersion. First, a limited number of dispersion curves are numerically calculated for a three-dimensional cell model by changing C_m and _i, and their amplitudes and relaxation times are determined by assuming a Cole-Cole function. Second, regression formulas are obtained from the values of and and then used for the determination of C_m and _i from the experimental and . This method was applied to the dielectric dispersion measured for rabbit erythrocytes (discocytes and echinocytes) and human erythrocytes (normocytes), and provided reasonable C_m and _i of the erythrocytes and excellent agreement between the theoretical and experimental dispersion curves.