BIOPHYSICAL THEORY AND MODELING |
A closed-form analytic expression for FRAP formula for the binding diffusion model
Minchul Kang 1* and Anne K Kenworthy 1
1 Vanderbilt School of Medicine
* To whom correspondence should be addressed. E-mail: minchul.kang{at}vanderbilt.edu.
Submitted on April 22, 2008
Revised on May 5, 2008
Accepted on 12 May 2008
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Abstract |
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One of most dominant methods cells use for a large class of cellular processes is reaction (or binding) diffusion kinetics, which are controlled by kinetic constants such as diffusion coefficients and on/off binding rate constants. Fluorescence Recovery After Photobleaching (FRAP) can be used to determine these kinetic constants in living cells. While an analytic expression for FRAP formulae for pure diffusion has been available for some time, an analytic FRAP formula for the binding diffusion model has not been reported yet. Here, we present an analytic FRAP formula for binding diffusion model in an explicit form allowing for diffusion of the bound complex for either a uniform circle laser profile or a Gaussian laser profile.
Key Words:
Analytic formula, Binding diffusion, FRAP
