SPECTROSCOPY IMAGING OTHER TECHNIQUES

From the Current Issue: January 15 2007; 92 (2) [Table of Contents]

Delphine Debarre and Emmanuel Beaurepaire

Quantitative Characterization of Biological Liquids for Third-Harmonic Generation Microscopy
Biophys. J. 2007. 92:603-612. [Abstract] [Full Text] [PDF]

S. Semrau and T. Schmidt

Particle Image Correlation Spectroscopy (PICS): Retrieving Nanometer-Scale Correlations from High-Density Single-Molecule Position Data
Biophys. J. 2007. 92:613-621. [Abstract] [Full Text] [PDF]

From Past Issues:

Nam Ki Lee, Achillefs N. Kapanidis, Hye Ran Koh, You Korlann, Sam On Ho, Younggyu Kim, Natalie Gassman, Seong Keun Kim, and Shimon Weiss

Three-Color Alternating-Laser Excitation of Single Molecules: Monitoring Multiple Interactions and Distances
Biophys. J. 2007. 92:303-312. [Abstract] [Full Text] [PDF]

Hugo Sanabria, Yoshihisa Kubota, and M. Neal Waxham

Multiple Diffusion Mechanisms Due to Nanostructuring in Crowded Environments
Biophys. J. 2007. 92:313-322. [Abstract] [Full Text] [PDF]

Balint Kintses, Zoltan Simon, Mate Gyimesi, Julia Toth, Balazs Jelinek, Csaba Niedetzky, Mihaly Kovacs, and Andras Malnasi-Csizmadia

Enzyme Kinetics above Denaturation Temperature: A Temperature-Jump/Stopped-Flow Apparatus
Biophys. J. 2006. 91:4605-4610. [Abstract] [Full Text] [PDF]

Jonathan W. D. Comeau, Santiago Costantino, and Paul W. Wiseman

A Guide to Accurate Fluorescence Microscopy Colocalization Measurements
Biophys. J. 2006. 91:4611-4622. [Abstract] [Full Text] [PDF]

Tennille Presley, Periannan Kuppusamy, Jay L. Zweier, and Govindasamy Ilangovan

Electron Paramagnetic Resonance Oximetry as a Quantitative Method to Measure Cellular Respiration: A Consideration of Oxygen Diffusion Interference
Biophys. J. 2006. 91:4623-4631. [Abstract] [Full Text] [PDF]

T. Roopa and G. V. Shivashankar

Direct Measurement of Local Chromatin Fluidity Using Optical Trap Modulation Force Spectroscopy
Biophys. J. 2006. 91:4632-4637. [Abstract] [Full Text] [PDF]

Daniel R. Sisan, Richard Arevalo, Catherine Graves, Ryan McAllister, and Jeffrey S. Urbach

Spatially Resolved Fluorescence Correlation Spectroscopy Using a Spinning Disk Confocal Microscope
Biophys. J. 2006. 91:4241-4252. [Abstract] [Full Text] [PDF]

John Peter Rickgauer, Derek N. Fuller, and Douglas E. Smith

DNA as a Metrology Standard for Length and Force Measurements with Optical Tweezers
Biophys. J. 2006. 91:4253-4257. [Abstract] [Full Text] [PDF]

Samuel T. Hess, Thanu P. K. Girirajan, and Michael D. Mason

Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
Biophys. J. 2006. 91:4258-4272. [Abstract] [Full Text] [PDF]

Lindsey N. Hillesheim, Yan Chen, and Joachim D. Muller

Dual-Color Photon Counting Histogram Analysis of mRFP1 and EGFP in Living Cells
Biophys. J. 2006. 91:4273-4284. [Abstract] [Full Text] [PDF]

Bogumil Zelent, Jane M. Vanderkooi, Ryan G. Coleman, Ignacy Gryczynski, and Zygmunt Gryczynski

Protonation of Excited State Pyrene-1-Carboxylate by Phosphate and Organic Acids in Aqueous Solution Studied by Fluorescence Spectroscopy
Biophys. J. 2006. 91:3864-3871. [Abstract] [Full Text] [PDF]

C. A. Bertrand, C. Laboisse, U. Hopfer, R. J. Bridges, and R. A. Frizzell

Methods for Detecting Internalized, FM 1-43 Stained Particles in Epithelial Cells and Monolayers
Biophys. J. 2006. 91:3872-3883. [Abstract] [Full Text] [PDF]

Mikhail Sergeev, Santiago Costantino, and Paul W. Wiseman

Measurement of Monomer-Oligomer Distributions via Fluorescence Moment Image Analysis
Biophys. J. 2006. 91:3884-3896. [Abstract] [Full Text] [PDF]

James D. Gwyer, Jingdong Zhang, Julea N. Butt, and Jens Ulstrup

Voltammetry and In Situ Scanning Tunneling Microscopy of Cytochrome c Nitrite Reductase on Au(111) Electrodes
Biophys. J. 2006. 91:3897-3906. [Abstract] [Full Text] [PDF]

Yu Ohsugi, Kenta Saito, Mamoru Tamura, and Masataka Kinjo

Lateral Mobility of Membrane-Binding Proteins in Living Cells Measured by Total Internal Reflection Fluorescence Correlation Spectroscopy
Biophys. J. 2006. 91:3456-3464. [Abstract] [Full Text] [PDF]

G. M. Akselrod, W. Timp, U. Mirsaidov, Q. Zhao, C. Li, R. Timp, K. Timp, P. Matsudaira, and G. Timp

Laser-Guided Assembly of Heterotypic Three-Dimensional Living Cell Microarrays
Biophys. J. 2006. 91:3465-3473. [Abstract] [Full Text] [PDF]

Uzoma M. Okorie and Scott L. Diamond

Matrix Protein Microarrays for Spatially and Compositionally Controlled Microspot Thrombosis under Laminar Flow
Biophys. J. 2006. 91:3474-3481. [Abstract] [Full Text] [PDF]

Michael C. Fink, Kenneth V. Adair, Marina G. Guenza, and Andrew H. Marcus

Translational Diffusion of Fluorescent Proteins by Molecular Fourier Imaging Correlation Spectroscopy
Biophys. J. 2006. 91:3482-3498. [Abstract] [Full Text] [PDF]

B. Christoffer Lagerholm, Laurel Averett, Gabriel E. Weinreb, Ken Jacobson, and Nancy L. Thompson

Analysis Method for Measuring Submicroscopic Distances with Blinking Quantum Dots
Biophys. J. 2006. 91:3050-3060. [Abstract] [Full Text] [PDF]

David L. Kolin, David Ronis, and Paul W. Wiseman

k-Space Image Correlation Spectroscopy: A Method for Accurate Transport Measurements Independent of Fluorophore Photophysics
Biophys. J. 2006. 91:3061-3075. [Abstract] [Full Text] [PDF]

Eduard Y. Chekmenev, Peter L. Gor'kov, Timothy A. Cross, Ali M. Alaouie, and Alex I. Smirnov

Flow-Through Lipid Nanotube Arrays for Structure-Function Studies of Membrane Proteins by Solid-State NMR Spectroscopy
Biophys. J. 2006. 91:3076-3084. [Abstract] [Full Text] [PDF]

Yen Sun, Wei-Liang Chen, Sung-Jan Lin, Shiou-Hwa Jee, Yang-Fang Chen, Ling-Chih Lin, Peter T. C. So, and Chen-Yuan Dong

Investigating Mechanisms of Collagen Thermal Denaturation by High Resolution Second-Harmonic Generation Imaging
Biophys. J. 2006. 91:2620-2625. [Abstract] [Full Text] [PDF]

J. Borejdo, Z. Gryczynski, N. Calander, P. Muthu, and I. Gryczynski

Application of Surface Plasmon Coupled Emission to Study of Muscle
Biophys. J. 2006. 91:2626-2635. [Abstract] [Full Text] [PDF]

Ziad Ganim and Andrei Tokmakoff

Spectral Signatures of Heterogeneous Protein Ensembles Revealed by MD Simulations of 2DIR Spectra
Biophys. J. 2006. 91:2636-2646. [Abstract] [Full Text] [PDF]

Christian Beier and Heinz-Jurgen Steinhoff

A Structure-Based Simulation Approach for Electron Paramagnetic Resonance Spectra Using Molecular and Stochastic Dynamics Simulations
Biophys. J. 2006. 91:2647-2664. [Abstract] [Full Text] [PDF]

Miklos S. Z. Kellermayer, Arpad Karsai, Andras Kengyel, Attila Nagy, Pasquale Bianco, Tamas Huber, Agnes Kulcsar, Csaba Niedetzky, Roger Proksch, and Laszlo Grama

Spatially and Temporally Synchronized Atomic Force and Total Internal Reflection Fluorescence Microscopy for Imaging and Manipulating Cells and Biomolecules
Biophys. J. 2006. 91:2665-2677. [Abstract] [Full Text] [PDF]

Brian P. Lynch, Al M. Hilton, and Garth J. Simpson

Nanoscale Dielectrophoretic Spectroscopy of Individual Immobilized Mammalian Blood Cells
Biophys. J. 2006. 91:2678-2686. [Abstract] [Full Text] [PDF]

Bin Wu, Yan Chen, and Joachim D. Muller

Dual-Color Time-Integrated Fluorescence Cumulant Analysis
Biophys. J. 2006. 91:2687-2698. [Abstract] [Full Text] [PDF]

Eeva-Liisa Karjalainen, Amelie Hardell, and Andreas Barth

Toward a General Method to Observe the Phosphate Groups of Phosphoenzymes with Infrared Spectroscopy
Biophys. J. 2006. 91:2282-2289. [Abstract] [Full Text] [PDF]

Fei Wu, Xueqian Zhang, Joseph Y. Cheung, Kebin Shi, Zhiwen Liu, Claire Luo, Stuart Yin, and Paul Ruffin

Frequency Division Multiplexed Multichannel High-Speed Fluorescence Confocal Microscope
Biophys. J. 2006. 91:2290-2296. [Abstract] [Full Text] [PDF]

Jonas Ries and Petra Schwille

Studying Slow Membrane Dynamics with Continuous Wave Scanning Fluorescence Correlation Spectroscopy
Biophys. J. 2006. 91:1915-1924. [Abstract] [Full Text] [PDF]

Ye Fang, Ann M. Ferrie, Norman H. Fontaine, John Mauro, and Jitendra Balakrishnan

Resonant Waveguide Grating Biosensor for Living Cell Sensing
Biophys. J. 2006. 91:1925-1940. [Abstract] [Full Text] [PDF]

Sean A. McKinney, Chirlmin Joo, and Taekjip Ha

Analysis of Single-Molecule FRET Trajectories Using Hidden Markov Modeling
Biophys. J. 2006. 91:1941-1951. [Abstract] [Full Text] [PDF]

Fei Yu and J. H. Prestegard

Structural Monitoring of Oligosaccharides through ¹³C Enrichment and NMR Observation of Acetyl Groups
Biophys. J. 2006. 91:1952-1959. [Abstract] [Full Text] [PDF]

E. S. Salnikov, D. A. Erilov, A. D. Milov, Yu. D. Tsvetkov, C. Peggion, F. Formaggio, C. Toniolo, J. Raap, and S. A. Dzuba

Location and Aggregation of the Spin-Labeled Peptide Trichogin GA IV in a Phospholipid Membrane as Revealed by Pulsed EPR
Biophys. J. 2006. 91:1532-1540. [Abstract] [Full Text] [PDF]

Richard J. Gilbert, Lee H. Magnusson, Vitaly J. Napadow, Thomas Benner, Ruopeng Wang, and Van J. Wedeen

Mapping Complex Myoarchitecture in the Bovine Tongue with Diffusion-Spectrum Magnetic Resonance Imaging
Biophys. J. 2006. 91:1014-1022. [Abstract] [Full Text] [PDF]

Larry J. Friedman, Johnson Chung, and Jeff Gelles

Viewing Dynamic Assembly of Molecular Complexes by Multi-Wavelength Single-Molecule Fluorescence
Biophys. J. 2006. 91:1023-1031. [Abstract] [Full Text] [PDF]

Ben Corry, Dylan Jayatilaka, Boris Martinac, and Paul Rigby

Determination of the Orientational Distribution and Orientation Factor for Transfer between Membrane-Bound Fluorophores using a Confocal Microscope
Biophys. J. 2006. 91:1032-1045. [Abstract] [Full Text] [PDF]

Ian R. Bates, Benedict Hebert, Yishan Luo, Jie Liao, Alexia I. Bachir, David L. Kolin, Paul W. Wiseman, and John W. Hanrahan

Membrane Lateral Diffusion and Capture of CFTR within Transient Confinement Zones
Biophys. J. 2006. 91:1046-1058. [Abstract] [Full Text] [PDF]

Yougang Mao and Yong Ba

Insight into the Binding of Antifreeze Proteins to Ice Surfaces via ¹³C Spin Lattice Relaxation Solid-State NMR
Biophys. J. 2006. 91:1059-1068. [Abstract] [Full Text] [PDF]

Ricardo R. Brau, Peter B. Tarsa, Jorge M. Ferrer, Peter Lee, and Matthew J. Lang

Interlaced Optical Force-Fluorescence Measurements for Single Molecule Biophysics
Biophys. J. 2006. 91:1069-1077. [Abstract] [Full Text] [PDF]

Ling Chin Hwang, Michael Gosch, Theo Lasser, and Thorsten Wohland

Simultaneous Multicolor Fluorescence Cross-Correlation Spectroscopy to Detect Higher Order Molecular Interactions Using Single Wavelength Laser Excitation
Biophys. J. 2006. 91:715-727. [Abstract] [Full Text] [PDF]

Xiaolin Nan, Eric O. Potma, and X. Sunney Xie

Nonperturbative Chemical Imaging of Organelle Transport in Living Cells with Coherent Anti-Stokes Raman Scattering Microscopy
Biophys. J. 2006. 91:728-735. [Abstract] [Full Text] [PDF]

Wangchen Wang, Deng Pan, Yang Song, Wenhan Liu, Lin Yang, and Huey W. Huang

Method of X-Ray Anomalous Diffraction for Lipid Structures
Biophys. J. 2006. 91:736-743. [Abstract] [Full Text] [PDF]

Hugo Sanabria, John H. Miller Jr., Andreas Mershin, Richard F. Luduena, Alexandre A. Kolomenski, Hans A. Schuessler, and Dimitri V. Nanopoulos

Impedance Spectroscopy of -ß Tubulin Heterodimer Suspensions
Biophys. J. 2006. 90:4644-4650. [Abstract] [Full Text] [PDF]

Patrick H. Brown and Peter Schuck

Macromolecular Size-and-Shape Distributions by Sedimentation Velocity Analytical Ultracentrifugation
Biophys. J. 2006. 90:4651-4661. [Abstract] [Full Text] [PDF]

Thomas P. Burghardt, Jon E. Charlesworth, Miriam F. Halstead, James E. Tarara, and Katalin Ajtai

In Situ Fluorescent Protein Imaging with Metal Film-Enhanced Total Internal Reflection Microscopy
Biophys. J. 2006. 90:4662-4671. [Abstract] [Full Text] [PDF]

Casey H. Londergan, Jianping Wang, Paul H. Axelsen, and Robin M. Hochstrasser

Two-Dimensional Infrared Spectroscopy Displays Signatures of Structural Ordering in Peptide Aggregates
Biophys. J. 2006. 90:4672-4685. [Abstract] [Full Text] [PDF]

Todd Sulchek, Raymond W. Friddle, and Aleksandr Noy

Strength of Multiple Parallel Biological Bonds
Biophys. J. 2006. 90:4686-4691. [Abstract] [Full Text] [PDF]

Elizabeth Rhoades, Trudy F. Ramlall, Watt W. Webb, and David Eliezer

Quantification of -Synuclein Binding to Lipid Vesicles Using Fluorescence Correlation Spectroscopy
Biophys. J. 2006. 90:4692-4700. [Abstract] [Full Text] [PDF]

Erin E. Wilson, Ayorinde Awonusi, Michael D. Morris, David H. Kohn, Mary M. J. Tecklenburg, and Larry W. Beck

Three Structural Roles for Water in Bone Observed by Solid-State NMR
Biophys. J. 2006. 90:3722-3731. [Abstract] [Full Text] [PDF]

Kiyoshi Kawai, Toru Suzuki, and Masaharu Oguni

Low-Temperature Glass Transitions of Quenched and Annealed Bovine Serum Albumin Aqueous Solutions
Biophys. J. 2006. 90:3732-3738. [Abstract] [Full Text] [PDF]

Itsushi Minoura and Etsuko Muto

Dielectric Measurement of Individual Microtubules Using the Electroorientation Method
Biophys. J. 2006. 90:3739-3748. [Abstract] [Full Text] [PDF]

K. T. Samiee, J. M. Moran-Mirabal, Y. K. Cheung, and H. G. Craighead

Zero Mode Waveguides for Single-Molecule Spectroscopy on Lipid Membranes
Biophys. J. 2006. 90:3288-3299. [Abstract] [Full Text] [PDF]

Ranieri Bizzarri, Caterina Arcangeli, Daniele Arosio, Fernanda Ricci, Paolo Faraci, Francesco Cardarelli, and Fabio Beltram

Development of a Novel GFP-based Ratiometric Excitation and Emission pH Indicator for Intracellular Studies
Biophys. J. 2006. 90:3300-3314. [Abstract] [Full Text] [PDF]

Martin J. Bishop, Blanca Rodriguez, James Eason, Jonathan P. Whiteley, Natalia Trayanova, and David J. Gavaghan

Synthesis of Voltage-Sensitive Optical Signals: Application to Panoramic Optical Mapping
Biophys. J. 2006. 90:2938-2945. [Abstract] [Full Text] [PDF]

Erik Goormaghtigh, Jean-Marie Ruysschaert, and Vincent Raussens

Evaluation of the Information Content in Infrared Spectra for Protein Secondary Structure Determination
Biophys. J. 2006. 90:2946-2957. [Abstract] [Full Text] [PDF]

Rob Zondervan, Florian Kulzer, Harmen van der Meer, Jos A. J. M. Disselhorst, and Michel Orrit

Laser-Driven Microsecond Temperature Cycles Analyzed by Fluorescence Polarization Microscopy
Biophys. J. 2006. 90:2958-2969. [Abstract] [Full Text] [PDF]

Thomas Braun, Natalija Backmann, Manuel Vogtli, Alexander Bietsch, Andreas Engel, Hans-Peter Lang, Christoph Gerber, and Martin Hegner

Conformational Change of Bacteriorhodopsin Quantitatively Monitored by Microcantilever Sensors
Biophys. J. 2006. 90:2970-2977. [Abstract] [Full Text] [PDF]

Soren Barsberg, Pavel Matousek, Mike Towrie, Henning Jorgensen, and Claus Felby

Lignin Radicals in the Plant Cell Wall Probed by Kerr-Gated Resonance Raman Spectroscopy
Biophys. J. 2006. 90:2978-2986. [Abstract] [Full Text] [PDF]

T. M. Dugdale, R. Dagastine, A. Chiovitti, and R. Wetherbee

Diatom Adhesive Mucilage Contains Distinct Supramolecular Assemblies of a Single Modular Protein
Biophys. J. 2006. 90:2987-2993. [Abstract] [Full Text] [PDF]

Yong-Bin Yan, Jun Zhang, Hua-Wei He, and Hai-Meng Zhou

Oligomerization and Aggregation of Bovine Pancreatic Ribonuclease A: Characteristic Events Observed by FTIR Spectroscopy
Biophys. J. 2006. 90:2525-2533. [Abstract] [Full Text] [PDF]

Tsai-Wen Chen, Bei-Jung Lin, Edgar Brunner, and Detlev Schild

In Situ Background Estimation in Quantitative Fluorescence Imaging
Biophys. J. 2006. 90:2534-2547. [Abstract] [Full Text] [PDF]

A. Delon, Y. Usson, J. Derouard, T. Biben, and C. Souchier

Continuous Photobleaching in Vesicles and Living Cells: A Measure of Diffusion and Compartmentation
Biophys. J. 2006. 90:2548-2562. [Abstract] [Full Text] [PDF]

Lei Jin, Andrew C. Millard, Joseph P. Wuskell, Xuemei Dong, Dianqing Wu, Heather A. Clark, and Leslie M. Loew

Characterization and Application of a New Optical Probe for Membrane Lipid Domains
Biophys. J. 2006. 90:2563-2575. [Abstract] [Full Text] [PDF]

Joseph Knab, Jing-Yin Chen, and Andrea Markelz

Hydration Dependence of Conformational Dielectric Relaxation of Lysozyme
Biophys. J. 2006. 90:2576-2581. [Abstract] [Full Text] [PDF]

F. v. Wegner, M. Both, and R. H. A. Fink

Automated Detection of Elementary Calcium Release Events Using the À Trous Wavelet Transform
Biophys. J. 2006. 90:2151-2163. [Abstract] [Full Text] [PDF]

Keiichi Kameyama and Allen P. Minton

Rapid Quantitative Characterization of Protein Interactions by Composition Gradient Static Light Scattering
Biophys. J. 2006. 90:2164-2169. [Abstract] [Full Text] [PDF]

James E. Shaw, Raquel F. Epand, Richard M. Epand, Zaiguo Li, Robert Bittman, and Christopher M. Yip

Correlated Fluorescence-Atomic Force Microscopy of Membrane Domains: Structure of Fluorescence Probes Determines Lipid Localization
Biophys. J. 2006. 90:2170-2178. [Abstract] [Full Text] [PDF]

Kaupo Palo, Ulo Mets, Vello Loorits, and Peet Kask

Calculation of Photon-Count Number Distributions via Master Equations
Biophys. J. 2006. 90:2179-2191. [Abstract] [Full Text] [PDF]

Anna Tourovskaia, T. Fettah Kosar, and Albert Folch

Local Induction of Acetylcholine Receptor Clustering in Myotube Cultures Using Microfluidic Application of Agrin
Biophys. J. 2006. 90:2192-2198. [Abstract] [Full Text] [PDF]

Marco Mank, Dierk F. Reiff, Nicola Heim, Michael W. Friedrich, Alexander Borst, and Oliver Griesbeck

A FRET-Based Calcium Biosensor with Fast Signal Kinetics and High Fluorescence Change
Biophys. J. 2006. 90:1790-1796. [Abstract] [Full Text] [PDF]

Seung-Cheol Lee, Jee-Hyun Cho, Daniel Mietchen, Young-Sook Kim, Kwan Soo Hong, Chulhyun Lee, Dongmin Kang, Ki Deok Park, Byong-Seok Choi, and Chaejoon Cheong

Subcellular In Vivo ¹H MR Spectroscopy of Xenopus laevis Oocytes
Biophys. J. 2006. 90:1797-1803. [Abstract] [Full Text] [PDF]

Marco V. Bayas, Andrew Leung, Evan Evans, and Deborah Leckband

Lifetime Measurements Reveal Kinetic Differences between Homophilic Cadherin Bonds
Biophys. J. 2006. 90:1385-1395. [Abstract] [Full Text] [PDF]

J. L. Swift, R. Heuff, and D. T. Cramb

A Two-Photon Excitation Fluorescence Cross-Correlation Assay for a Model Ligand-Receptor Binding System Using Quantum Dots
Biophys. J. 2006. 90:1396-1410. [Abstract] [Full Text] [PDF]

Georg E. Fantner, Emin Oroudjev, Georg Schitter, Laura S. Golde, Philipp Thurner, Marquesa M. Finch, Patricia Turner, Thomas Gutsmann, Daniel E. Morse, Helen Hansma, and Paul K. Hansma

Sacrificial Bonds and Hidden Length: Unraveling Molecular Mesostructures in Tough Materials
Biophys. J. 2006. 90:1411-1418. [Abstract] [Full Text] [PDF]

Emmanuel Margeat, Achillefs N. Kapanidis, Philip Tinnefeld, You Wang, Jayanta Mukhopadhyay, Richard H. Ebright, and Shimon Weiss

Direct Observation of Abortive Initiation and Promoter Escape within Single Immobilized Transcription Complexes
Biophys. J. 2006. 90:1419-1431. [Abstract] [Full Text] [PDF]

Igor Sokolov, Venkatesh Subba-Rao, and Linda A. Luck

Change in Rigidity in the Activated Form of the Glucose/Galactose Receptor from Escherichia coli: A Phenomenon that Will Be Key to the Development of Biosensors
Biophys. J. 2006. 90:1055-1063. [Abstract] [Full Text] [PDF]

Francisco J. Alvarez-Leefmans, Jose J. Herrera-Perez, Martin S. Marquez, and Victor M. Blanco

Simultaneous Measurement of Water Volume and pH in Single Cells Using BCECF and Fluorescence Imaging Microscopy
Biophys. J. 2006. 90:608-618. [Abstract] [Full Text] [PDF]

Vladimir P. Zharov, Kelly E. Mercer, Elena N. Galitovskaya, and Mark S. Smeltzer

Photothermal Nanotherapeutics and Nanodiagnostics for Selective Killing of Bacteria Targeted with Gold Nanoparticles
Biophys. J. 2006. 90:619-627. [Abstract] [Full Text] [PDF]

David L. Kolin, Santiago Costantino, and Paul W. Wiseman

Sampling Effects, Noise, and Photobleaching in Temporal Image Correlation Spectroscopy
Biophys. J. 2006. 90:628-639. [Abstract] [Full Text] [PDF]

Michael J. Higgins, John E. Sader, and Suzanne P. Jarvis

Frequency Modulation Atomic Force Microscopy Reveals Individual Intermediates Associated with each Unfolded I27 Titin Domain
Biophys. J. 2006. 90:640-647. [Abstract] [Full Text] [PDF]

James W. Chan, Douglas S. Taylor, Theodore Zwerdling, Stephen M. Lane, Ko Ihara, and Thomas Huser

Micro-Raman Spectroscopy Detects Individual Neoplastic and Normal Hematopoietic Cells
Biophys. J. 2006. 90:648-656. [Abstract] [Full Text] [PDF]

Christopher Deufel and Michelle D. Wang

Detection of Forces and Displacements along the Axial Direction in an Optical Trap
Biophys. J. 2006. 90:657-667. [Abstract] [Full Text] [PDF]

L. Stirling Churchman, Henrik Flyvbjerg, and James A. Spudich

A Non-Gaussian Distribution Quantifies Distances Measured with Fluorescence Localization Techniques
Biophys. J. 2006. 90:668-671. [Abstract] [Full Text] [PDF]

Thomas C. Kaufmann, Andreas Engel, and Herve-W. Remigy

A Novel Method for Detergent Concentration Determination
Biophys. J. 2006. 90:310-317. [Abstract] [Full Text] [PDF]

Valeria Levi, Anna S. Serpinskaya, Enrico Gratton, and Vladimir Gelfand

Organelle Transport along Microtubules in Xenopus Melanophores: Evidence for Cooperation between Multiple Motors
Biophys. J. 2006. 90:318-327. [Abstract] [Full Text] [PDF]

Sergey Plotnikov, Vaibhav Juneja, Ariel B. Isaacson, William A. Mohler, and Paul J. Campagnola

Optical Clearing for Improved Contrast in Second Harmonic Generation Imaging of Skeletal Muscle
Biophys. J. 2006. 90:328-339. [Abstract] [Full Text] [PDF]

Eric J. Hustedt, Richard A. Stein, Latsavongsakda Sethaphong, Suzanne Brandon, Zheng Zhou, and Susan C. DeSensi

Dipolar Coupling between Nitroxide Spin Labels: The Development and Application of a Tether-in-a-Cone Model
Biophys. J. 2006. 90:340-356. [Abstract] [Full Text] [PDF]

T. M. Dugdale, R. Dagastine, A. Chiovitti, P. Mulvaney, and R. Wetherbee

Single Adhesive Nanofibers from a Live Diatom Have the Signature Fingerprint of Modular Proteins
Biophys. J. 2005. 89:4252-4260. [Abstract] [Full Text] [PDF]

Sergi Garcia-Manyes, Gerard Oncins, and Fausto Sanz

Effect of Temperature on the Nanomechanics of Lipid Bilayers Studied by Force Spectroscopy
Biophys. J. 2005. 89:4261-4274. [Abstract] [Full Text] [PDF]

Valeria Levi, QiaoQiao Ruan, Matthew Plutz, Andrew S. Belmont, and Enrico Gratton

Chromatin Dynamics in Interphase Cells Revealed by Tracking in a Two-Photon Excitation Microscope
Biophys. J. 2005. 89:4275-4285. [Abstract] [Full Text] [PDF]

Alessandro Esposito, Hans C. Gerritsen, and Fred S. Wouters

Fluorescence Lifetime Heterogeneity Resolution in the Frequency Domain by Lifetime Moments Analysis
Biophys. J. 2005. 89:4286-4299. [Abstract] [Full Text] [PDF]

E. J. Hukkanen, J. A. Wieland, A. Gewirth, D. E. Leckband, and R. D. Braatz

Multiple-Bond Kinetics from Single-Molecule Pulling Experiments: Evidence for Multiple NCAM Bonds
Biophys. J. 2005. 89:3434-3445. [Abstract] [Full Text] [PDF]

P. S. Dittrich, S. P. Schafer, and P. Schwille

Characterization of the Photoconversion on Reaction of the Fluorescent Protein Kaede on the Single-Molecule Level
Biophys. J. 2005. 89:3446-3455. [Abstract] [Full Text] [PDF]

A. Ponti, A. Matov, M. Adams, S. Gupton, C. M. Waterman-Storer, and G. Danuser

Periodic Patterns of Actin Turnover in Lamellipodia and Lamellae of Migrating Epithelial Cells Analyzed by Quantitative Fluorescent Speckle Microscopy
Biophys. J. 2005. 89:3456-3469. [Abstract] [Full Text] [PDF]

Hsiao-Wei Liu, Gonzalo Cosa, Christy F. Landes, Yining Zeng, Brandie J. Kovaleski, Daniel G. Mullen, George Barany, Karin Musier-Forsyth, and Paul F. Barbara

Single-Molecule FRET Studies of Important Intermediates in the Nucleocapsid-Protein-Chaperoned Minus-Strand Transfer Step in HIV-1 Reverse Transcription
Biophys. J. 2005. 89:3470-3479. [Abstract] [Full Text] [PDF]

Li Li, Haifeng Wang, and Ji-Xin Cheng

Quantitative Coherent Anti-Stokes Raman Scattering Imaging of Lipid Distribution in Coexisting Domains
Biophys. J. 2005. 89:3480-3490. [Abstract] [Full Text] [PDF]

Lindsey N. Hillesheim and Joachim D. Muller

The Dual-Color Photon Counting Histogram with Non-Ideal Photodetectors
Biophys. J. 2005. 89:3491-3507. [Abstract] [Full Text] [PDF]

Barbara K. Muller, Evgeny Zaychikov, Christoph Brauchle, and Don C. Lamb

Pulsed Interleaved Excitation
Biophys. J. 2005. 89:3508-3522. [Abstract] [Full Text] [PDF]

Eric B. Brauns and R. Brian Dyer

Time-Resolved Infrared Spectroscopy of RNA Folding
Biophys. J. 2005. 89:3523-3530. [Abstract] [Full Text] [PDF]

Alvaro Sanchez-Andres, Yan Chen, and Joachim D. Muller

Molecular Brightness Determined from a Generalized Form of Mandel's Q-Parameter
Biophys. J. 2005. 89:3531-3547. [Abstract] [Full Text] [PDF]

Bin Wu and Joachim D. Muller

Time-Integrated Fluorescence Cumulant Analysis in Fluorescence Fluctuation Spectroscopy
Biophys. J. 2005. 89:2721-2735. [Abstract] [Full Text] [PDF]

Christopher Thaler, Srinagesh V. Koushik, Paul S. Blank, and Steven S. Vogel

Quantitative Multiphoton Spectral Imaging and Its Use for Measuring Resonance Energy Transfer
Biophys. J. 2005. 89:2736-2749. [Abstract] [Full Text] [PDF]

Keiko Tawa and Kenichi Morigaki

Substrate-Supported Phospholipid Membranes Studied by Surface Plasmon Resonance and Surface Plasmon Fluorescence Spectroscopy
Biophys. J. 2005. 89:2750-2758. [Abstract] [Full Text] [PDF]

Caroline M. Ajo-Franklin, Prasad V. Ganesan, and Steven G. Boxer

Variable Incidence Angle Fluorescence Interference Contrast Microscopy for Z-Imaging Single Objects
Biophys. J. 2005. 89:2759-2769. [Abstract] [Full Text] [PDF]

Tobias Kohl, Elke Haustein, and Petra Schwille

Determining Protease Activity In Vivo by Fluorescence Cross-Correlation Analysis
Biophys. J. 2005. 89:2770-2782. [Abstract] [Full Text] [PDF]

B. Bonanni, A. S. M. Kamruzzahan, A. R. Bizzarri, C. Rankl, H. J. Gruber, P. Hinterdorfer, and S. Cannistraro

Single Molecule Recognition between Cytochrome C 551 and Gold-Immobilized Azurin by Force Spectroscopy
Biophys. J. 2005. 89:2783-2791. [Abstract] [Full Text] [PDF]

Jan K. Rainey and Brian D. Sykes

Optimizing Oriented Planar-Supported Lipid Samples for Solid-State Protein NMR
Biophys. J. 2005. 89:2792-2805. [Abstract] [Full Text] [PDF]

Janusz Pyka, Jan Ilnicki, Christian Altenbach, Wayne L. Hubbell, and Wojciech Froncisz

Accessibility and Dynamics of Nitroxide Side Chains in T4 Lysozyme Measured by Saturation Recovery EPR
Biophys. J. 2005. 89:2059-2068. [Abstract] [Full Text] [PDF]

Elmar Thews, Margarita Gerken, Reiner Eckert, Johannes Zapfel, Carsten Tietz, and Jorg Wrachtrup

Cross Talk Free Fluorescence Cross Correlation Spectroscopy in Live Cells
Biophys. J. 2005. 89:2069-2076. [Abstract] [Full Text] [PDF]

Attila Nagy, Jianrong Wu, and Keith M. Berland

Observation Volumes and -Factors in Two-Photon Fluorescence Fluctuation Spectroscopy
Biophys. J. 2005. 89:2077-2090. [Abstract] [Full Text] [PDF]

Randolf Jordan, Edward A. Lemke, and Jurgen Klingauf

Visualization of Synaptic Vesicle Movement in Intact Synaptic Boutons Using Fluorescence Fluctuation Spectroscopy
Biophys. J. 2005. 89:2091-2102. [Abstract] [Full Text] [PDF]

Christian Altenbach, Wojciech Froncisz, Roy Hemker, Hassane Mchaourab, and Wayne L. Hubbell

Accessibility of Nitroxide Side Chains: Absolute Heisenberg Exchange Rates from Power Saturation EPR
Biophys. J. 2005. 89:2103-2112. [Abstract] [Full Text] [PDF]

Henrike Heise, Sorin Luca, Bert L. de Groot, Helmut Grubmuller, and Marc Baldus

Probing Conformational Disorder in Neurotensin by Two-Dimensional Solid-State NMR and Comparison to Molecular Dynamics Simulations
Biophys. J. 2005. 89:2113-2120. [Abstract] [Full Text] [PDF]

Santiago Costantino, Jonathan W. D. Comeau, David L. Kolin, and Paul W. Wiseman

Accuracy and Dynamic Range of Spatial Image Correlation and Cross-Correlation Spectroscopy
Biophys. J. 2005. 89:1251-1260. [Abstract] [Full Text] [PDF]

Joseph N. Forkey, Margot E. Quinlan, and Yale E. Goldman

Measurement of Single Macromolecule Orientation by Total Internal Reflection Fluorescence Polarization Microscopy
Biophys. J. 2005. 89:1261-1271. [Abstract] [Full Text] [PDF]

Seth Blumberg, Arivalagan Gajraj, Matthew W. Pennington, and Jens-Christian Meiners

Three-Dimensional Characterization of Tethered Microspheres by Total Internal Reflection Fluorescence Microscopy
Biophys. J. 2005. 89:1272-1281. [Abstract] [Full Text] [PDF]

V. Kurkal, R. M. Daniel, John L. Finney, M. Tehei, R. V. Dunn, and Jeremy C. Smith

Enzyme Activity and Flexibility at Very Low Hydration
Biophys. J. 2005. 89:1282-1287. [Abstract] [Full Text] [PDF]

Joseph P. Skinner, Yan Chen, and Joachim D. Muller

Position-Sensitive Scanning Fluorescence Correlation Spectroscopy
Biophys. J. 2005. 89:1288-1301. [Abstract] [Full Text] [PDF]

Y. H. Sniekers and C. C. van Donkelaar

Determining Diffusion Coefficients in Inhomogeneous Tissues Using Fluorescence Recovery after Photobleaching
Biophys. J. 2005. 89:1302-1307. [Abstract] [Full Text] [PDF]

Hanbin Mao, J. Ricardo Arias-Gonzalez, Steven B. Smith, Ignacio Tinoco Jr., and Carlos Bustamante

Temperature Control Methods in a Laser Tweezers System
Biophys. J. 2005. 89:1308-1316. [Abstract] [Full Text] [PDF]

Michelle A. Digman, Claire M. Brown, Parijat Sengupta, Paul W. Wiseman, Alan R. Horwitz, and Enrico Gratton

Measuring Fast Dynamics in Solutions and Cells with a Laser Scanning Microscope
Biophys. J. 2005. 89:1317-1327. [Abstract] [Full Text] [PDF]

Daniel Hilger, Heinrich Jung, Etana Padan, Christoph Wegener, Klaus-Peter Vogel, Heinz-Jurgen Steinhoff, and Gunnar Jeschke

Assessing Oligomerization of Membrane Proteins by Four-Pulse DEER: pH-Dependent Dimerization of NhaA Na⁺/H⁺ Antiporter of E. coli
Biophys. J. 2005. 89:1328-1338. [Abstract] [Full Text] [PDF]

Elmar Schmalzlin, Joost T. van Dongen, Ingo Klimant, Bettina Marmodee, Martin Steup, Joachim Fisahn, Peter Geigenberger, and Hans-Gerd Lohmannsroben

An Optical Multifrequency Phase-Modulation Method Using Microbeads for Measuring Intracellular Oxygen Concentrations in Plants
Biophys. J. 2005. 89:1339-1345. [Abstract] [Full Text] [PDF]

Marc Schneider, Sara Barozzi, Ilaria Testa, Mario Faretta, and Alberto Diaspro

Two-Photon Activation and Excitation Properties of PA-GFP in the 720–920-nm Region
Biophys. J. 2005. 89:1346-1352. [Abstract] [Full Text] [PDF]

C. Bertocchi, A. Ravasio, S. Bernet, G. Putz, P. Dietl, and T. Haller

Optical Measurement of Surface Tension in a Miniaturized Air-Liquid Interface and its Application in Lung Physiology
Biophys. J. 2005. 89:1353-1361. [Abstract] [Full Text] [PDF]

J. Korlach, C. Reichle, T. Muller, T. Schnelle, and W. W. Webb

Trapping, Deformation, and Rotation of Giant Unilamellar Vesicles in Octode Dielectrophoretic Field Cages
Biophys. J. 2005. 89:554-562. [Abstract] [Full Text] [PDF]

Joshua Manor, Ziad Khattari, Tim Salditt, and Isaiah T. Arkin

Disorder Influence on Linear Dichroism Analyses of Smectic Phases
Biophys. J. 2005. 89:563-571. [Abstract] [Full Text] [PDF]

Natallia Makarava, Alexander Parfenov, and Ilia V. Baskakov

Water-Soluble Hybrid Nanoclusters with Extra Bright and Photostable Emissions: A New Tool for Biological Imaging
Biophys. J. 2005. 89:572-580. [Abstract] [Full Text] [PDF]

Haifeng Wang, Yan Fu, Phyllis Zickmund, Riyi Shi, and Ji-Xin Cheng

Coherent Anti-Stokes Raman Scattering Imaging of Axonal Myelin in Live Spinal Tissues
Biophys. J. 2005. 89:581-591. [Abstract] [Full Text] [PDF]

Xiaomin Bin, Sarah L. Horswell, and Jacek Lipkowski

Electrochemical and PM-IRRAS Studies of the Effect of Cholesterol on the Structure of a DMPC Bilayer Supported at an Au (111) Electrode Surface, Part 1: Properties of the Acyl Chains
Biophys. J. 2005. 89:592-604. [Abstract] [Full Text] [PDF]

Christian Eggeling, Peet Kask, Dirk Winkler, and Stefan Jager

Rapid Analysis of Förster Resonance Energy Transfer by Two-Color Global Fluorescence Correlation Spectroscopy: Trypsin Proteinase Reaction
Biophys. J. 2005. 89:605-618. [Abstract] [Full Text] [PDF]

Julie Dam, Carlos A. Velikovsky, Roy A. Mariuzza, Claus Urbanke, and Peter Schuck

Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation Coefficient Distributions c(s)
Biophys. J. 2005. 89:619-634. [Abstract] [Full Text] [PDF]

L. J. Kaufman, C. P. Brangwynne, K. E. Kasza, E. Filippidi, V. D. Gordon, T. S. Deisboeck, and D. A. Weitz

Glioma Expansion in Collagen I Matrices: Analyzing Collagen Concentration-Dependent Growth and Motility Patterns
Biophys. J. 2005. 89:635-650. [Abstract] [Full Text] [PDF]

Julie Dam and Peter Schuck

Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Sedimentation Coefficient Distributions c(s) and Asymptotic Boundary Profiles from Gilbert-Jenkins Theory
Biophys. J. 2005. 89:651-666. [Abstract] [Full Text] [PDF]

Kurt W. Short, Susan Carpenter, James P. Freyer, and Judith R. Mourant

Raman Spectroscopy Detects Biochemical Changes Due to Proliferation in Mammalian Cell Cultures
Biophys. J. 2005. 88:4274-4288. [Abstract] [Full Text] [PDF]

Martynas Gavutis, Suman Lata, Peter Lamken, Pia Muller, and Jacob Piehler

Lateral Ligand-Receptor Interactions on Membranes Probed by Simultaneous Fluorescence-Interference Detection
Biophys. J. 2005. 88:4289-4302. [Abstract] [Full Text] [PDF]

Alan I. Lee and James P. Brody

Single-Molecule Enzymology of Chymotrypsin Using Water-in-Oil Emulsion
Biophys. J. 2005. 88:4303-4311. [Abstract] [Full Text] [PDF]

Georg Hildenbrand, Alexander Rapp, Udo Spori, Christian Wagner, Christoph Cremer, and Michael Hausmann

Nano-Sizing of Specific Gene Domains in Intact Human Cell Nuclei by Spatially Modulated Illumination Light Microscopy
Biophys. J. 2005. 88:4312-4318. [Abstract] [Full Text] [PDF]

Sally A. Kim, Katrin G. Heinze, Kirsten Bacia, M. Neal Waxham, and Petra Schwille

Two-Photon Cross-Correlation Analysis of Intracellular Reactions with Variable Stoichiometry
Biophys. J. 2005. 88:4319-4336. [Abstract] [Full Text] [PDF]

Michel Vincent, Beatrice de Foresta, and Jacques Gallay

Nanosecond Dynamics of a Mimicked Membrane-Water Interface Observed by Time-Resolved Stokes Shift of LAURDAN
Biophys. J. 2005. 88:4337-4350. [Abstract] [Full Text] [PDF]

Kerstin Jacobsen, Shirley Oga, Wayne L. Hubbell, and Thomas Risse

Determination of the Orientation of T4 Lysozyme Vectorially Bound to a Planar-Supported Lipid Bilayer Using Site-Directed Spin Labeling
Biophys. J. 2005. 88:4351-4365. [Abstract] [Full Text] [PDF]

Yan Chen, Li-Na Wei, and Joachim D. Muller

Unraveling Protein-Protein Interactions in Living Cells with Fluorescence Fluctuation Brightness Analysis
Biophys. J. 2005. 88:4366-4377. [Abstract] [Full Text] [PDF]

Linda C. Pravinata, Yumin You, and Richard D. Ludescher

Erythrosin B Phosphorescence Monitors Molecular Mobility and Dynamic Site Heterogeneity in Amorphous Sucrose
Biophys. J. 2005. 88:3551-3561. [Abstract] [Full Text] [PDF]

Eric Girard, Richard Kahn, Mohamed Mezouar, Anne-Claire Dhaussy, Tianwei Lin, John E. Johnson, and Roger Fourme

The First Crystal Structure of a Macromolecular Assembly under High Pressure: CpMV at 330 MPa
Biophys. J. 2005. 88:3562-3571. [Abstract] [Full Text] [PDF]

L. H. Pope, M. L. Bennink, K. A. van Leijenhorst-Groener, D. Nikova, J. Greve, and J. F. Marko

Single Chromatin Fiber Stretching Reveals Physically Distinct Populations of Disassembly Events
Biophys. J. 2005. 88:3572-3583. [Abstract] [Full Text] [PDF]

Akiko Masuda, Kiminori Ushida, and Takayuki Okamoto

New Fluorescence Correlation Spectroscopy Enabling Direct Observation of Spatiotemporal Dependence of Diffusion Constants as an Evidence of Anomalous Transport in Extracellular Matrices
Biophys. J. 2005. 88:3584-3591. [Abstract] [Full Text] [PDF]

Anjali Dike and Sudha M. Cowsik

Membrane-Induced Structure of Scyliorhinin I: A Dual NK1/NK2 Agonist
Biophys. J. 2005. 88:3592-3600. [Abstract] [Full Text] [PDF]

Benedict Hebert, Santiago Costantino, and Paul W. Wiseman

Spatiotemporal Image Correlation Spectroscopy (STICS) Theory, Verification, and Application to Protein Velocity Mapping in Living CHO Cells
Biophys. J. 2005. 88:3601-3614. [Abstract] [Full Text] [PDF]

Man Liu, Eeva-Liisa Karjalainen, and Andreas Barth

Use of Helper Enzymes for ADP Removal in Infrared Spectroscopic Experiments: Application to Ca²⁺-ATPase
Biophys. J. 2005. 88:3615-3624. [Abstract] [Full Text] [PDF]

Evgeny Kobrinsky, Donald E. Mager, Sarah A. Bentil, Shin-ichi Murata, Darrell R. Abernethy, and Nikolai M. Soldatov

Identification of Plasma Membrane Macro- and Microdomains from Wavelet Analysis of FRET Microscopy
Biophys. J. 2005. 88:3625-3634. [Abstract] [Full Text] [PDF]

Brian Mohlenhoff, Melissa Romeo, Max Diem, and Bayden R. Wood

Mie-Type Scattering and Non-Beer-Lambert Absorption Behavior of Human Cells in Infrared Microspectroscopy
Biophys. J. 2005. 88:3635-3640. [Abstract] [Full Text] [PDF]

Candice S. Klug, Theodore G. Camenisch, Wayne L. Hubbell, and James S. Hyde

Multiquantum EPR Spectroscopy of Spin-Labeled Arrestin K267C at 35 GHz
Biophys. J. 2005. 88:3641-3647. [Abstract] [Full Text] [PDF]

Valeria Levi, QiaoQiao Ruan, and Enrico Gratton

3-D Particle Tracking in a Two-Photon Microscope: Application to the Study of Molecular Dynamics in Cells
Biophys. J. 2005. 88:2919-2928. [Abstract] [Full Text] [PDF]

Jeremy D. Wilson, Chad E. Bigelow, David J. Calkins, and Thomas H. Foster

Light Scattering from Intact Cells Reports Oxidative-Stress-Induced Mitochondrial Swelling
Biophys. J. 2005. 88:2929-2938. [Abstract] [Full Text] [PDF]

Nam Ki Lee, Achillefs N. Kapanidis, You Wang, Xavier Michalet, Jayanta Mukhopadhyay, Richard H. Ebright, and Shimon Weiss

Accurate FRET Measurements within Single Diffusing Biomolecules Using Alternating-Laser Excitation
Biophys. J. 2005. 88:2939-2953. [Abstract] [Full Text] [PDF]

A. Arcovito, D. C. Lamb, G. U. Nienhaus, J. L. Hazemann, M. Benfatto, and S. Della Longa

Light-Induced Relaxation of Photolyzed Carbonmonoxy Myoglobin: A Temperature-Dependent X-Ray Absorption Near-Edge Structure (XANES) Study
Biophys. J. 2005. 88:2954-2964. [Abstract] [Full Text] [PDF]

Carine Galli Marxer, Mary L. Kraft, Peter K. Weber, Ian D. Hutcheon, and Steven G. Boxer

Supported Membrane Composition Analysis by Secondary Ion Mass Spectrometry with High Lateral Resolution
Biophys. J. 2005. 88:2965-2975. [Abstract] [Full Text] [PDF]

Ikuko Koyama-Honda, Ken Ritchie, Takahiro Fujiwara, Ryota Iino, Hideji Murakoshi, Rinshi S. Kasai, and Akihiro Kusumi

Fluorescence Imaging for Monitoring the Colocalization of Two Single Molecules in Living Cells
Biophys. J. 2005. 88:2126-2136. [Abstract] [Full Text] [PDF]

Anthony H. B. de Vries, Bea E. Krenn, Roel van Driel, and Johannes S. Kanger

Micro Magnetic Tweezers for Nanomanipulation Inside Live Cells
Biophys. J. 2005. 88:2137-2144. [Abstract] [Full Text] [PDF]

K. T. Samiee, M. Foquet, L. Guo, E. C. Cox, and H. G. Craighead

-Repressor Oligomerization Kinetics at High Concentrations Using Fluorescence Correlation Spectroscopy in Zero-Mode Waveguides
Biophys. J. 2005. 88:2145-2153. [Abstract] [Full Text] [PDF]

Z. Vivian Feng, Tighe A. Spurlin, and Andrew A. Gewirth

Direct Visualization of Asymmetric Behavior in Supported Lipid Bilayers at the Gel-Fluid Phase Transition
Biophys. J. 2005. 88:2154-2164. [Abstract] [Full Text] [PDF]

Emily C. Rothstein, Stefanie Carroll, Christian A. Combs, Paul D. Jobsis, and Robert S. Balaban

Skeletal Muscle NAD(P)H Two-Photon Fluorescence Microscopy In Vivo: Topology and Optical Inner Filters
Biophys. J. 2005. 88:2165-2176. [Abstract] [Full Text] [PDF]

Yan Chen, Mohac Tekmen, Lindsey Hillesheim, Joseph Skinner, Bin Wu, and Joachim D. Muller

Dual-Color Photon-Counting Histogram
Biophys. J. 2005. 88:2177-2192. [Abstract] [Full Text] [PDF]

Adam Rosenthal and Joel Voldman

Dielectrophoretic Traps for Single-Particle Patterning
Biophys. J. 2005. 88:2193-2205. [Abstract] [Full Text] [PDF]

Rebecca M. Williams, Warren R. Zipfel, and Watt W. Webb

Interpreting Second-Harmonic Generation Images of Collagen I Fibrils
Biophys. J. 2005. 88:1377-1386. [Abstract] [Full Text] [PDF]

Jeffrey R. Kuhn and Thomas D. Pollard

Real-Time Measurements of Actin Filament Polymerization by Total Internal Reflection Fluorescence Microscopy
Biophys. J. 2005. 88:1387-1402. [Abstract] [Full Text] [PDF]

James H. Werner, Hong Cai, Richard A. Keller, and Peter M. Goodwin

Exonuclease I Hydrolyzes DNA with a Distribution of Rates
Biophys. J. 2005. 88:1403-1412. [Abstract] [Full Text] [PDF]

Krishnananda Chattopadhyay, Saveez Saffarian, Elliot L. Elson, and Carl Frieden

Measuring Unfolding of Proteins in the Presence of Denaturant Using Fluorescence Correlation Spectroscopy
Biophys. J. 2005. 88:1413-1422. [Abstract] [Full Text] [PDF]

Harald Janovjak, Daniel J. Muller, and Andrew D. L. Humphris

Molecular Force Modulation Spectroscopy Revealing the Dynamic Response of Single Bacteriorhodopsins
Biophys. J. 2005. 88:1423-1431. [Abstract] [Full Text] [PDF]

Geza Berecki, Jan G. Zegers, Arie O. Verkerk, Zahurul A. Bhuiyan, Berend de Jonge, Marieke W. Veldkamp, Ronald Wilders, and Antoni C. G. van Ginneken

HERG Channel (Dys)function Revealed by Dynamic Action Potential Clamp Technique
Biophys. J. 2005. 88:566-578. [Abstract] [Full Text] [PDF]

Peter M. Kasson, Johannes B. Huppa, Michelle Krogsgaard, Mark M. Davis, and Axel T. Brunger

Quantitative Imaging of Lymphocyte Membrane Protein Reorganization and Signaling
Biophys. J. 2005. 88:579-589. [Abstract] [Full Text] [PDF]

Mary K. Cowman, Chiara Spagnoli, Dina Kudasheva, Min Li, Ansil Dyal, Sonoko Kanai, and Endre A. Balazs

Extended, Relaxed, and Condensed Conformations of Hyaluronan Observed by Atomic Force Microscopy
Biophys. J. 2005. 88:590-602. [Abstract] [Full Text] [PDF]

Marco Plomp, Terrance J. Leighton, Katherine E. Wheeler, and Alexander J. Malkin

The High-Resolution Architecture and Structural Dynamics of Bacillus Spores
Biophys. J. 2005. 88:603-608. [Abstract] [Full Text] [PDF]

Richard K. P. Benninger, Bjorn Onfelt, Mark A. A. Neil, Daniel M. Davis, and Paul M. W. French

Fluorescence Imaging of Two-Photon Linear Dichroism: Cholesterol Depletion Disrupts Molecular Orientation in Cell Membranes
Biophys. J. 2005. 88:609-622. [Abstract] [Full Text] [PDF]

Thierry Savin and Patrick S. Doyle

Static and Dynamic Errors in Particle Tracking Microrheology
Biophys. J. 2005. 88:623-638. [Abstract] [Full Text] [PDF]

Dessy N. Nikova, Lisa H. Pope, Martin L. Bennink, Kirsten A. van Leijenhorst-Groener, Kees van der Werf, and Jan Greve

Unexpected Binding Motifs for Subnucleosomal Particles Revealed by Atomic Force Microscopy
Biophys. J. 2004. 87:4135-4145. [Abstract] [Full Text] [PDF]

Hilmar Gugel, Jorg Bewersdorf, Stefan Jakobs, Johann Engelhardt, Rafael Storz, and Stefan W. Hell

Cooperative 4Pi Excitation and Detection Yields Sevenfold Sharper Optical Sections in Live-Cell Microscopy
Biophys. J. 2004. 87:4146-4152. [Abstract] [Full Text] [PDF]

Sanjay Tyagi and Osama Alsmadi

Imaging Native ß-Actin mRNA in Motile Fibroblasts
Biophys. J. 2004. 87:4153-4162. [Abstract] [Full Text] [PDF]

Nada N. Boustany, Yien-Che Tsai, Bryan Pfister, Wilsaan M. Joiner, George A. Oyler, and Nitish V. Thakor

BCL-x_L-Dependent Light Scattering by Apoptotic Cells
Biophys. J. 2004. 87:4163-4171. [Abstract] [Full Text] [PDF]

Christian Blum, Alfred J. Meixner, and Vinod Subramaniam

Room Temperature Spectrally Resolved Single-Molecule Spectroscopy Reveals New Spectral Forms and Photophysical Versatility of Aequorea Green Fluorescent Protein Variants
Biophys. J. 2004. 87:4172-4179. [Abstract] [Full Text] [PDF]

Y. Hwu, W. L. Tsai, H. M. Chang, H. I. Yeh, P. C. Hsu, Y. C. Yang, Y. T. Su, H. L. Tsai, G. M. Chow, P. C. Ho, S. C. Li, H. O. Moser, P. Yang, S. K. Seol, C. C. Kim, J. H. Je, E. Stefanekova, A. Groso, and G. Margaritondo

Imaging Cells and Tissues with Refractive Index Radiology
Biophys. J. 2004. 87:4180-4187. [Abstract] [Full Text] [PDF]

Coralie Alonso, Tim Alig, Joonsung Yoon, Frank Bringezu, Heidi Warriner, and Joseph A. Zasadzinski

More Than a Monolayer: Relating Lung Surfactant Structure and Mechanics to Composition
Biophys. J. 2004. 87:4188-4202. [Abstract] [Full Text] [PDF]

E. L. Botvinick, V. Venugopalan, J. V. Shah, L. H. Liaw, and M. W. Berns

Controlled Ablation of Microtubules Using a Picosecond Laser
Biophys. J. 2004. 87:4203-4212. [Abstract] [Full Text] [PDF]

V. Volkov and P. Hamm

A Two-Dimensional Infrared Study of Localization, Structure, and Dynamics of a Dipeptide in Membrane Environment
Biophys. J. 2004. 87:4213-4225. [Abstract] [Full Text] [PDF]

M. Guthold, W. Liu, B. Stephens, S. T. Lord, R. R. Hantgan, D. A. Erie, R. M. Taylor Jr., and R. Superfine

Visualization and Mechanical Manipulations of Individual Fibrin Fibers Suggest that Fiber Cross Section Has Fractal Dimension 1.3
Biophys. J. 2004. 87:4226-4236. [Abstract] [Full Text] [PDF]

Boris G. Dzikovski, Petr P. Borbat, and Jack H. Freed

Spin-Labeled Gramicidin A: Channel Formation and Dissociation
Biophys. J. 2004. 87:3504-3517. [Abstract] [Full Text] [PDF]

Matthias Weiss, Markus Elsner, Fredrik Kartberg, and Tommy Nilsson

Anomalous Subdiffusion Is a Measure for Cytoplasmic Crowding in Living Cells
Biophys. J. 2004. 87:3518-3524. [Abstract] [Full Text] [PDF]

Anatoli Ianoul, Melissa Street, Donna Grant, John Pezacki, Rod S. Taylor, and Linda J. Johnston

Near-Field Scanning Fluorescence Microscopy Study of Ion Channel Clusters in Cardiac Myocyte Membranes
Biophys. J. 2004. 87:3525-3535. [Abstract] [Full Text] [PDF]

Hazen P. Babcock, Chen Chen, and Xiaowei Zhuang

Using Single-Particle Tracking to Study Nuclear Trafficking of Viral Genes
Biophys. J. 2004. 87:2749-2758. [Abstract] [Full Text] [PDF]

Gonzalo Cosa, Elizabeth J. Harbron, Yining Zeng, Hsiao-Wei Liu, Donald B. O'Connor, Chie Eta-Hosokawa, Karin Musier-Forsyth, and Paul F. Barbara

Secondary Structure and Secondary Structure Dynamics of DNA Hairpins Complexed with HIV-1 NC Protein
Biophys. J. 2004. 87:2759-2767. [Abstract] [Full Text] [PDF]

Brett Shoelson, Emilios K. Dimitriadis, Hongxue Cai, Bechara Kachar, and Richard S. Chadwick

Evidence and Implications of Inhomogeneity in Tectorial Membrane Elasticity
Biophys. J. 2004. 87:2768-2777. [Abstract] [Full Text] [PDF]

Aikaterini Zoumi, Xiao Lu, Ghassan S. Kassab, and Bruce J. Tromberg

Imaging Coronary Artery Microstructure Using Second-Harmonic and Two-Photon Fluorescence Microscopy
Biophys. J. 2004. 87:2778-2786. [Abstract] [Full Text] [PDF]

Alexa L. Mattheyses, Adam D. Hoppe, and Daniel Axelrod

Polarized Fluorescence Resonance Energy Transfer Microscopy
Biophys. J. 2004. 87:2787-2797. [Abstract] [Full Text] [PDF]

Burak Okumus, Timothy J. Wilson, David M. J. Lilley, and Taekjip Ha

Vesicle Encapsulation Studies Reveal that Single Molecule Ribozyme Heterogeneities Are Intrinsic
Biophys. J. 2004. 87:2798-2806. [Abstract] [Full Text] [PDF]

S. Pelet, M. J. R. Previte, L. H. Laiho, and P. T. C. So

A Fast Global Fitting Algorithm for Fluorescence Lifetime Imaging Microscopy Based on Image Segmentation
Biophys. J. 2004. 87:2807-2817. [Abstract] [Full Text] [PDF]

H. Wang, R. Bash, J. G. Yodh, G. Hager, S. M. Lindsay, and D. Lohr

Using Atomic Force Microscopy to Study Nucleosome Remodeling on Individual Nucleosomal Arrays in Situ
Biophys. J. 2004. 87:1964-1971. [Abstract] [Full Text] [PDF]

Rajalakshmi Nambiar, Arivalagan Gajraj, and Jens-Christian Meiners

All-Optical Constant-Force Laser Tweezers
Biophys. J. 2004. 87:1972-1980. [Abstract] [Full Text] [PDF]

Cordula M. Stroh, Andreas Ebner, Manfred Geretschlager, Gunter Freudenthaler, Ferry Kienberger, A. S. M. Kamruzzahan, Sandra J. Smith-Gill, Hermann J. Gruber, and Peter Hinterdorfer

Simultaneous Topography and Recognition Imaging Using Force Microscopy
Biophys. J. 2004. 87:1981-1990. [Abstract] [Full Text] [PDF]

Dongdong Li, Jun Xiong, Anlian Qu, and Tao Xu

Three-Dimensional Tracking of Single Secretory Granules in Live PC12 Cells
Biophys. J. 2004. 87:1991-2001. [Abstract] [Full Text] [PDF]

Rachel Marrington, Timothy R. Dafforn, David J. Halsall, and Alison Rodger

Micro-Volume Couette Flow Sample Orientation for Absorbance and Fluorescence Linear Dichroism
Biophys. J. 2004. 87:2002-2012. [Abstract] [Full Text] [PDF]

Qiaoqiao Ruan, Melanie A. Cheng, Moshe Levi, Enrico Gratton, and William W. Mantulin

Spatial-Temporal Studies of Membrane Dynamics: Scanning Fluorescence Correlation Spectroscopy (SFCS)
Biophys. J. 2004. 87:1260-1267. [Abstract] [Full Text] [PDF]

Alena M. Lieto and Nancy L. Thompson

Total Internal Reflection with Fluorescence Correlation Spectroscopy: Nonfluorescent Competitors
Biophys. J. 2004. 87:1268-1278. [Abstract] [Full Text] [PDF]

Rhonda Dzakpasu and Daniel Axelrod

Dynamic Light Scattering Microscopy. A Novel Optical Technique to Image Submicroscopic Motions. I: Theory
Biophys. J. 2004. 87:1279-1287. [Abstract] [Full Text] [PDF]

Rhonda Dzakpasu and Daniel Axelrod

Dynamic Light Scattering Microscopy. A Novel Optical Technique to Image Submicroscopic Motions. II: Experimental Applications
Biophys. J. 2004. 87:1288-1297. [Abstract] [Full Text] [PDF]

Dakota Watson, Norbert Hagen, Jonathan Diver, Philippe Marchand, and Mirianas Chachisvilis

Elastic Light Scattering from Single Cells: Orientational Dynamics in Optical Trap
Biophys. J. 2004. 87:1298-1306. [Abstract] [Full Text] [PDF]

Dimitri Ivanov, Nicolas Dubreuil, Vincent Raussens, Jean-Marie Ruysschaert, and Erik Goormaghtigh

Evaluation of the Ordering of Membranes in Multilayer Stacks Built on an ATR-FTIR Germanium Crystal with Atomic Force Microscopy: The Case of the H⁺,K⁺-ATPase-containing Gastric Tubulovesicle Membranes
Biophys. J. 2004. 87:1307-1315. [Abstract] [Full Text] [PDF]

Marisa Martin-Fernandez, Samantha V. Longshaw, Ian Kirby, George Santis, Mark J. Tobin, David T. Clarke, and Gareth R. Jones

Adenovirus Type-5 Entry and Disassembly Followed in Living Cells by FRET, Fluorescence Anisotropy, and FLIM
Biophys. J. 2004. 87:1316-1327. [Abstract] [Full Text] [PDF]

Sungchul Hohng, Chirlmin Joo, and Taekjip Ha

Single-Molecule Three-Color FRET
Biophys. J. 2004. 87:1328-1337. [Abstract] [Full Text] [PDF]

Ganesh Shanmugam and Prasad L. Polavarapu

Structure of Aß(25–35) Peptide in Different Environments
Biophys. J. 2004. 87:622-630. [Abstract] [Full Text] [PDF]

Bernd Kuhn, Peter Fromherz, and Winfried Denk

High Sensitivity of Stark-Shift Voltage-Sensing Dyes by One- or Two-Photon Excitation Near the Red Spectral Edge
Biophys. J. 2004. 87:631-639. [Abstract] [Full Text] [PDF]

L. Kreplak, J. Doucet, P. Dumas, and F. Briki

New Aspects of the -Helix to ß-Sheet Transition in Stretched Hard -Keratin Fibers
Biophys. J. 2004. 87:640-647. [Abstract] [Full Text] [PDF]

Oksana Kostyuk and Robert A. Brown

Novel Spectroscopic Technique for In Situ Monitoring of Collagen Fibril Alignment in Gels
Biophys. J. 2004. 87:648-655. [Abstract] [Full Text] [PDF]

Dehong Hu and H. Peter Lu

Placing Single-Molecule T4 Lysozyme Enzymes on a Bacterial Cell Surface: Toward Probing Single-Molecule Enzymatic Reaction in Living Cells
Biophys. J. 2004. 87:656-661. [Abstract] [Full Text] [PDF]

Orsolya Toke, W. Lee Maloy, Sung Joon Kim, Jack Blazyk, and Jacob Schaefer

Secondary Structure and Lipid Contact of a Peptide Antibiotic in Phospholipid Bilayers by REDOR
Biophys. J. 2004. 87:662-674. [Abstract] [Full Text] [PDF]

Orsolya Toke, R. D. O'Connor, Thomas K. Weldeghiorghis, W. Lee Maloy, Ralf W. Glaser, Anne S. Ulrich, and Jacob Schaefer

Structure of (KIAGKIA)₃ Aggregates in Phospholipid Bilayers by Solid-State NMR
Biophys. J. 2004. 87:675-687. [Abstract] [Full Text] [PDF]

Shi-Wei Chu, Szu-Yu Chen, Gia-Wei Chern, Tsung-Han Tsai, Yung-Chih Chen, Bai-Ling Lin, and Chi-Kuang Sun

Studies of ⁽²⁾/⁽³⁾ Tensors in Submicron-Scaled Bio-Tissues by Polarization Harmonics Optical Microscopy
Biophys. J. 2004. 86:3914-3922. [Abstract] [Full Text] [PDF]

Tomasz Zal and Nicholas R. J. Gascoigne

Photobleaching-Corrected FRET Efficiency Imaging of Live Cells
Biophys. J. 2004. 86:3923-3939. [Abstract] [Full Text] [PDF]

Eric J. Hustedt and Albert H. Beth

High Field/High Frequency Saturation Transfer Electron Paramagnetic Resonance Spectroscopy: Increased Sensitivity to Very Slow Rotational Motions
Biophys. J. 2004. 86:3940-3950. [Abstract] [Full Text] [PDF]

Ibon Iloro, Rosana Chehin, Felix M. Goni, Maria A. Pajares, and Jose-Luis R. Arrondo

Methionine Adenosyltransferase -Helix Structure Unfolds at Lower Temperatures than ß-Sheet: A 2D-IR Study
Biophys. J. 2004. 86:3951-3958. [Abstract] [Full Text] [PDF]

Thommey P. Thomas, Mon Thiri Myaing, Jing Yong Ye, Kimberly Candido, Alina Kotlyar, James Beals, Peter Cao, Balazs Keszler, Anil K. Patri, Theodore B. Norris, and James R. Baker Jr.

Detection and Analysis of Tumor Fluorescence Using a Two-Photon Optical Fiber Probe
Biophys. J. 2004. 86:3959-3965. [Abstract] [Full Text] [PDF]

Rikard Blunck, Dorine M. Starace, Ana M. Correa, and Francisco Bezanilla

Detecting Rearrangements of Shaker and NaChBac in Real-Time with Fluorescence Spectroscopy in Patch-Clamped Mammalian Cells
Biophys. J. 2004. 86:3966-3980. [Abstract] [Full Text] [PDF]

Joachim D. Muller

Cumulant Analysis in Fluorescence Fluctuation Spectroscopy
Biophys. J. 2004. 86:3981-3992. [Abstract] [Full Text] [PDF]

Sylvain V. Costes, Dirk Daelemans, Edward H. Cho, Zachary Dobbin, George Pavlakis, and Stephen Lockett

Automatic and Quantitative Measurement of Protein-Protein Colocalization in Live Cells
Biophys. J. 2004. 86:3993-4003. [Abstract] [Full Text] [PDF]

M. T. Valentine, Z. E. Perlman, M. L. Gardel, J. H. Shin, P. Matsudaira, T. J. Mitchison, and D. A. Weitz

Colloid Surface Chemistry Critically Affects Multiple Particle Tracking Measurements of Biomaterials
Biophys. J. 2004. 86:4004-4014. [Abstract] [Full Text] [PDF]

Lucas P. Watkins and Haw Yang

Information Bounds and Optimal Analysis of Dynamic Single Molecule Measurements
Biophys. J. 2004. 86:4015-4029. [Abstract] [Full Text] [PDF]

Michael L. Gostkowski, Richard Allen, Matthew L. Plenert, Eric Okerberg, Mary Jane Gordon, and Jason B. Shear

Multiphoton-Excited Serotonin Photochemistry
Biophys. J. 2004. 86:3223-3229. [Abstract] [Full Text] [PDF]

Karin Bettinger, Alexander Prutsch, Karsten Vogtt, and Mathias Lubben

Noninvasive Auto-Photoreduction Used as a Tool for Studying Structural Changes in Heme-Copper Oxidases by FTIR Spectroscopy
Biophys. J. 2004. 86:3230-3240. [Abstract] [Full Text] [PDF]

S. Magazu, G. Maisano, F. Migliardo, and C. Mondelli

Mean-Square Displacement Relationship in Bioprotectant Systems by Elastic Neutron Scattering
Biophys. J. 2004. 86:3241-3249. [Abstract] [Full Text] [PDF]

Angelo Demuro and Ian Parker

Imaging the Activity and Localization of Single Voltage-Gated Ca²⁺ Channels by Total Internal Reflection Fluorescence Microscopy
Biophys. J. 2004. 86:3250-3259. [Abstract] [Full Text] [PDF]

Itamar Kass, Eyal Arbely, and Isaiah T. Arkin

Modeling Sample Disorder in Site-Specific Dichroism Studies of Uniaxial Systems
Biophys. J. 2004